Animal Cell Biotechnology by Roland Wagner, Hansjörg Hauser

By Roland Wagner, Hansjörg Hauser

This booklet introduces the elemental ideas and sensible software of recommendations utilized in the scalable creation of proteins and virus-based gene-therapeutic items with animal mobilephone cultures. The e-book studies a large spectrum of topics suitable to biologics construction, together with the construction of mobilephone strains, a survey of useful genomics for mobilephone traces and techniques, and advances in upstream and downstream approach improvement. it really is an important reference for all these operating within the biotechnology box either from a tutorial and business point of view.

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26] Yew PR, Berk AJ. Inhibition of p53 transactivation required for transformation by adenovirus early 1B protein. Nature 1992;357:82-5. [27] Sweet BH, Hilleman MR. V. 40. Proceedings of the Society for Experimental Biology and Medicine 1960;105:420-7. [28] Pipas JM. SV40: Cell transformation and tumorigenesis. Virology 2009;384:294-303. [29] Kierstead TD, Tevethia MJ. Association of p53 binding and immortalization of primary C57BL/6 mouse embryo fibroblasts by using simian virus 40 T-antigen mutants bearing internal overlapping deletion mutations.

Importantly, these cells are able to differentiate into stratified epithelia in vitro. This, however, happens at a slower rate than with normal primary human keratinocytes [133]. Recently, a novel spontaneously immortalized human keratinocyte cell line, NIKS, was described. NIKS cells are immortal and also nontumorigenic. Importantly, they form a fully stratified squamous epithelium in vitro when cultivated in organotypic cultures. NIKS cells were isolated from juvenile foreskin keratinocytes and cultivated for the cell line generation on MEF feeders.

A Transgenic Mouse Marking Live Replicating Cells Reveals In Vivo Transcriptional Program of Proliferation. Developmental Cell 2012;23:681-90. [52] Tegtmeyer P, Schwartz M, Collins JK, et al. Regulation of tumor antigen synthesis by simain virus 40 gene A. Journal of Virology 1975;16:168-78. [53] Jat PS, Sharp PA. Cell lines established by a temperature-sensitive simian virus 40 large-Tantigen gene are growth restricted at the nonpermissive temperature. Molecular and Cellular Biology 1989;9:1672-81.

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